Search results for "Genus and species identification"

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Improvement of a rapid direct blood culture microbial identification protocol using MALDI-TOF MS and performance comparison with SepsiTyper kit

2018

Fast diagnosis of pathogens is critical to guarantee the most adequate therapy for infections; bacterial culture methods, which constitute the actual gold standard, are precise and sensitive but rather slow. Today, new methods have been made available to enable faster diagnosis, with the Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) technique being the most promising. Even if simpler and faster than traditional bacterial culture methods, analysis of positive blood cultures via MALDI-TOF MS requires a preliminary extraction process of samples. In this study, we compared two extraction protocols for bacterial identification directly from positive …

0301 basic medicineMicrobiology (medical)Time FactorsComputer science030106 microbiologyBacteremiaClinical diagnostic laboratorySensitivity and SpecificityMicrobiology03 medical and health sciencesSpecies SpecificitymedicineHumansBlood cultureOverall performanceMolecular BiologyProtocol (science)Bacteriological TechniquesChromatographyBacteriamedicine.diagnostic_testDiagnostic Tests RoutineGold standard (test)Matrix-assisted laser desorption/ionizationIdentification (information)Blood CulturePathogens identificationSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationPerformance comparisonCosts and Cost AnalysisGenus and species identificationMatrix- assisted laser desorption ionization time of flight mass spectrometryJournal of Microbiological Methods
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